pullorum strain h495 Search Results


h. pullorum strain h495 δcdt  (Inserm Transfert)
90
Inserm Transfert h. pullorum strain h495 δcdt
Bacterial strains and cell lines.
H. Pullorum Strain H495 δcdt, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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h. pullorum strain h495 δcdt - by Bioz Stars, 2026-02
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Bacterial strains and cell lines.

Journal: PLoS Pathogens

Article Title: Cytolethal distending toxin induces the formation of transient messenger-rich ribonucleoprotein nuclear invaginations in surviving cells

doi: 10.1371/journal.ppat.1007921

Figure Lengend Snippet: Bacterial strains and cell lines.

Article Snippet: H . pullorum strain H495 ΔCDT , INSERM U1053, Bordeaux, France , CDT - , T6SS + , [ ] , [ ] .

Techniques: Plasmid Preparation

(A ) Images of liver Hep3B and colon SW480 cells following a 72h coculture. Cells were stained with fluorescent primary and secondary antibodies targeting UNR (green) and DAPI to counterstain the nucleus (blue). Fluorescent staining was observed using wide field fluorescence imaging . Yellow and white arrowheads indicate UNR-NR and cells undergoing mitosis, respectively. (B) Quantification of UNR-NR positive nuclei (%). At least 200 cells were counted for each experiment. Data represent the mean of triplicates in 1 representative experiment out of 3. ** p = 0.0003 and *** p<0.0001. (C) Coculture experiments with liver Hep3B were also performed using a 0.4-μm Transwell system. * p<0.0154 and *** p<0.0001 ΔCDT, CDT-knockout mutant strain; ΔCol., bacterial artificial chromosome; Col., pks genomic island encoding colibactin; DAPI, 4′, 6′-diamidino-2-phenylindol; E . c ., Escherichia coli; H . h ., Helicobacter hepaticus; NI, non-infected; WT, wild-type H . hepaticus strain 3B1.

Journal: PLoS Pathogens

Article Title: Cytolethal distending toxin induces the formation of transient messenger-rich ribonucleoprotein nuclear invaginations in surviving cells

doi: 10.1371/journal.ppat.1007921

Figure Lengend Snippet: (A ) Images of liver Hep3B and colon SW480 cells following a 72h coculture. Cells were stained with fluorescent primary and secondary antibodies targeting UNR (green) and DAPI to counterstain the nucleus (blue). Fluorescent staining was observed using wide field fluorescence imaging . Yellow and white arrowheads indicate UNR-NR and cells undergoing mitosis, respectively. (B) Quantification of UNR-NR positive nuclei (%). At least 200 cells were counted for each experiment. Data represent the mean of triplicates in 1 representative experiment out of 3. ** p = 0.0003 and *** p<0.0001. (C) Coculture experiments with liver Hep3B were also performed using a 0.4-μm Transwell system. * p<0.0154 and *** p<0.0001 ΔCDT, CDT-knockout mutant strain; ΔCol., bacterial artificial chromosome; Col., pks genomic island encoding colibactin; DAPI, 4′, 6′-diamidino-2-phenylindol; E . c ., Escherichia coli; H . h ., Helicobacter hepaticus; NI, non-infected; WT, wild-type H . hepaticus strain 3B1.

Article Snippet: H . pullorum strain H495 ΔCDT , INSERM U1053, Bordeaux, France , CDT - , T6SS + , [ ] , [ ] .

Techniques: Staining, Fluorescence, Imaging, Knock-Out, Mutagenesis, Infection